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. 2020 Jan 10;11:213. doi: 10.1038/s41467-019-14001-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a GSEA was performed to analyze the pathways affected by HuR depletion in eWAT. Scatterplot depicts the relationship between the p-value and normalized enrichment score (NES) of 50 “Hallmark” gene sets in MSigDB. Significant biological pathways are labeled in red. b GSEA in adipogenesis pathway. c Real-time PCR to confirm the expression of genes for white fat and adipogenesis (Ctrl, n = 6; HuR-FKO, n = 7). d Real-time PCR to confirm the expression of genes for lipogenesis and glucose uptake (Ctrl, n = 6; HuR-FKO, n = 7). e GSEA in interferon gamma response and f inflammatory response pathway. At the bottom of each panel shows a heatmap of representative genes found within the leading edge-subset of the biological pathway. The color intensity represents median centered gene expression (FPKM) with red and blue representing highly and lowly expressed genes, respectively. g Real-time PCR to confirm the expression of genes involved in inflammation. n = 10 per group. Error bars are mean ± SEM. Statistical significance was determined by Student’s t-test; *p < 0.05. Source data are provided as a Source Data file.