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. 2020 Jan 10;11:213. doi: 10.1038/s41467-019-14001-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a GSEA was performed to analyze the pathways affected by HuR depletion in iWAT. Scatterplot depicts the relationship between the p-value and NES score of 50 “Hallmark” gene sets in MSigDB. b GSEA on the biological pathway of adipogenesis, c fatty acid metabolism, and d oxidative phosphorylation. e Real-time PCR to confirm the expression of genes for adipogenesis, lipogenesis, and f BAT-selective and beige fat markers (Ctrl, n = 6; HuR-FKO, n = 9). g Western blot was performed to examine the protein level of Cidea and Ucp1 in iWAT from HuR-FKO and Ctrl mice. h The regulated pathways in HuR-FKO iWAT and eWAT are displayed in a heatmap. The color represents the NES for each pathway. Error bars are mean ± SEM. Statistical significance was determined by Student’s t-test; *p < 0.05.