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. 2020 Jan 10;11:208. doi: 10.1038/s41467-019-14104-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Relative abundances of ascarosides in root exudates of Arabidopsis treated with 50 and 1000 nM ascr#18, as determined LC-MS. Data are average ± SEM (n = 6), n.d. = not detected. b Arabidopsis wildtype and acx1 acx5 were treated with 50 nM ascr#18 or ascr#9 for 48 h before transfer into 12-well plates containing Pluronic F-127 gel with ~200 freshly hatched M. incognita J2 larvae. Larvae touching the terminal part of roots were counted at 6 h after seedling transfer. Data are average ± s.d. (n = 12). c A small volume (10 µL) of ascr#18, ascr#9, or ascr#18/ascr#9 solutions was placed on one side of a 6-cm Petri dish with mock solutions on the other side. Subsequently, ~100 freshly hatched M. incognita J2 larvae were placed in the center of the plate. Larvae in the indicated scoring areas were counted after 4 h. d Attraction index for different concentrations of ascr#18, ascr#9, or ascr#18/ascr#9 mixtures measured using the layout shown in c. Data are mean ± SEM (n = 12). Adjusted p-values in Fig. 5b were calculated by two-way ANOVA followed by Tukey multiple comparisons post hoc test. For Fig. 5d, adjusted p-values were calculated by one-way ANOVA followed by Tukey multiple comparisons post hoc test. Source data are provided as a data file.