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. 2020 Jan 10;11:194. doi: 10.1038/s41467-019-14040-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Surface conservation analysis (green, variable; purple, conserved) of CUB domain. The calcium-binding site is circled in magenta. D134 and F135 are indicated by arrows. b D134 and F135 were both mutated to alanines through homologous recombination of a 150 bp ssODN containing a 5 bp mutation (red nucleotides). c Genotyping assay for the gpr126^stl464 lesion. The 5 bp mutation introduces a BstUI restriction enzyme binding site. d 4 dpf wild-type larva compared to e 4 dpf gpr126^{stl464/stl464} larva with puffy ears (arrowheads). Scale bars (black) represent 100 μm. f 4 dpf wild-type larvae express mbp throughout the posterior lateral line nerve (PLLn, arrowhead), whereas g 4 dpf gpr126^{stl464/stl464} larva lack mbp expression along the PLLn (arrowhead). Scale bars (black) represent 100 μm. Asterisks indicate CNS.