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. 2020 Jan 10;10:142. doi: 10.1038/s41598-019-56705-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IFC measurements of U937 cells at different frequencies using various PBS: Sucrose solution ratios. Minimal differences between necrotic and viable cells were obtained at a ratio of 7:3. A clear separation between the two cell populations was found at ratios of 2:3 and 1:4 (PBS: 0.28 M sucrose solution).