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. 2020 Jan 10;10:142. doi: 10.1038/s41598-019-56705-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NM-300K acute effect measured by IFC. The highest concentration of NM-300K (spherical Ag, 100 μg/mL) added for 10 and 30 min before the measurement induced acute toxicity in U937 cells. An overlay of four separate measurements is shown: viable cells (red), 10 min NM-300K exposure (blue), 30 min NM-300K exposure (purple), and necrotic cells (green).