Table 2.

Advantages and limitations of IFC.

Advantages	Limitations
• Label-free	• New assay: time for optimization, validation, and protocol development necessary
• Green alternative to classical assays. No toxic labelling reagents/buffers necessary	• Experience and training necessary
• Robust results: >10.000 cells/sample	• No information about the mechanism of toxicity
• Multiparametric readout possible
• Fast
• Less prone to nano-specific interferences
• Various channel-sizes to adjust sensitivity
• Increased high-throughput potential (auto sampler and barcode reader)
• Portable equipment