Fig. 3. Cold sensitivity caused by the siz1-2 mutation was not recovered by SIZ1 with mutation in the PHD finger.

a Three-week-old plants were incubated at 4 °C for 1 week for cold acclimation. Cold-acclimated plants were exposed for 4 h at − 6 °C. Following freezing treatment, plants were incubated at 24 °C for 1 week. Photographs are representative of WT, siz1-2, and siz1-2 transformed with SIZ1 variants. b Survival was determined in 27 plants after freezing treatment at − 6 °C. Data are the mean ± SD calculated from four or more independent experiments. Asterisks indicate a statistical difference from the siz1-2 plants (p < 0.05) as determined by Student’s t test.