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. 2020 Jan 10;10:126. doi: 10.1038/s41598-019-57022-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aqueous humor dynamics of IOP-elevated eyes. (A) IOP record of implanted eye of an anesthetized animal during outflow facility measurement. The bar indicates one duty cycle of the pump, which turned on and off to hold IOP at different levels above the resting IOP (15 mmHg). (B) Fluid outflow rate (F) at different IOP set points for the implanted (filled symbols) and non-implanted (unfilled symbols) eyes of animals exposed to 2 weeks (left), 4 weeks (middle), and 9 weeks (right) of IOP elevation. F was determined from measured duty cycles in A. Lines are regression fits to estimate the data slope, which determines conventional outflow facility (C). C, Measured C for the implanted and non-implanted eye of all animals in each exposure group.