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. 2020 Jan 10;11:215. doi: 10.1038/s41467-019-13916-6

Fig. 5. iPSC-derived HIOs grow in the absence of mesenchymal support.

Fig. 5

a Experimental schematic of MF and MC directed differentiations. b Light microscopy representative micrographs of merge images from BU1CG-derived HIOs cultured under MC vs MF conditions (scale bar = 100 μm, representative of n = 3 differentiations). c Representative fluorescent micrographs of HIOs derived using the MC vs MF Protocol stained for the mesenchymal marker Vimentin along with Cdx2 (scale bar = 50 μm, representative of n = 5 organoids from n = 3 differentiations). d Flow Cytometry of single-cell suspensions from HIOs differentiated using the MC vs MF protocol stained with the epithelial marker EpCAM. e Comparison of the % of EPCAM+ cells as measured by flow cytometry in HIOs cultured in different media conditions at day 54 of differentiation (paired Student’s t-test, *p < 0.05). f Flow cytometry for EpCAM expression of cells at days 6, 8, and 10 of both MC and MF differentiations. g UMAP representation of EpCAM expression at days 6 and 13 of differentiation by sc-RNAseq.