Table 1.
Primer sequences, annealing temperature and product size for MSP of target genes.
| Genes | Forward primer (5′ → 3′) | Annealing temperature(oC) | Product size (bp) | |
|---|---|---|---|---|
| CDKN2A | M | F:TTATTAGAGGGTGGGGCGGATCGC | 62 | 150 |
| R:GACCCCGAACCGCGACCGTAA | ||||
| U | F:TTATTAGAGGGTGGGGTGGATTGT | 62 | 151 | |
| R:CAACCCCAAACCACAACCATAA | ||||
| hMLH1 | M | F:ACGTAGACGTTTTATTAGGGTCGC | 60 | 118 |
| R:CCTCATCGTAACTACCCGCG | ||||
| U | F:TTTTGATGTAGATGTTTTATTAGGGTTGT | 60 | 124 | |
| R:ACCACCTCATCATAACTACCCACA | ||||
| MGMT | M | F:TTTCGACGTTCGTAGGTTTTCGC | 53 | 81 |
| R:GCACTCTTCCGAAAACGAAACG | ||||
| U | F:TTTGTGTTTTGATGTTTGTAGGTTTTTGT | 53 | 93 | |
| R:AACTCCACACTCTTCCAAAAACAAAACA | ||||
| CSF2 | M | F: TGATTATTTAGGGAAAAGGTTTATC | 56 | 105 |
| R: ATAACCACAAAATACCAAAAAAACG | ||||
| U | F: ATTATTTAGGGAAAAGGTTTATTGT | 60 | 104 | |
| R: AATAACCACAAAATACCAAAAAAACA | ||||
| DIS3L2 | M | F: GTCGTAGTTGAATCGTCGATTAC | 54 | 134 |
| R: TTACTAAAAAAAATACTCTTCCGAA | ||||
| U | F: GTTGTAGTTGAATTGTTGATTATGA | 55 | 134 | |
| R: TTACTAAAAAAAATACTCTTCCAAA | ||||
MSP, methylation-specific polymerase chain reaction; CDKN2A, cyclin-dependent kinase inhibitor 2A; MLH1, mutL homolog 1; MGMT, O-6-methylguanine DNA methyltransferase; CSF2, colony stimulating factor 2; DIS3L2, DIS3 mitotic control homolog (S. cerevisiae)-like 2; M, methylation; U, unmethylation.