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. 2020 Jan 10;11:192. doi: 10.1038/s41467-019-14037-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Immunoblotting of E-cadherin with the E-cad-Ab-QD probe and standard E-cadherin antibodies using different amount of A549 cells. b Fluorescence intensity curves of E-cadherin from different amount of cells. c Immunofluorescence microscopy of A549 cells stained with the E-cad-Ab-QD probe, the E-cad-Ab-QD probe with HNO₃ solution, and pure QDs. Scale bar, 25 μm. d Response currents of the electrochemical biosensor measured with or without A549 cells by DPV. e Optimization of the incubation time for the E-cad-QD probe. f Optimization of the E-cad-Ab-QD probe concentration for detecting E-cadherin. Source data are provided as a Source Data file.