Fig. 7. The degradation of ZmNSA1 confers transcriptional upregulation of MHA2 and MHA4.

a Phylogenetic tree of PM-H⁺-ATPase proteins from maize (MHA1-13) and Arabidopsis (AHA1-11). The phylogenetic tree was constructed using MEGA6⁴⁷. b The transcript levels of 13 MHAs in the root tissues. The figure was generated from the data downloaded from https://www.maizegdb.org. c The effect of saline-alkaline stress (100 mM NaHCO₃ for 6 hours) on the transcript levels of MHAs in the root tissues. Data were expressed as transcript levels relative to the MHA1 transcription under control condition. d–g The transcript levels of MHA2 and MHA4 in the root tissues (genotypes and treatments as indicated). h The effect of NaHCO₃ (100 mM) and LaCl₃ (5 mM) treatments on the transcript levels of MHA2 and MHA4. For c–h, 2-weeks-old plants were subjected to the indicate treatment, and then the root tissues were collected for analyzing the transcript levels of MHAs. Data in c–g were means ± s.d. of three independent experiments. Source data underlying Fig. 7c–h are provided as a Source Data file.