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. 2020 Jan 10;10:115. doi: 10.1038/s41598-019-56809-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Principal Component Analysis loading plot showing the correlation between metabolites and principal components 1 and 2. All 34 identified metabolites as well as four unknown components that were selected in the classification and regression tree analysis are displayed in the plot. Citrate appears twice as the CART analysis selected two different citrate peaks as components of importance in differentiating BRD and non-BRD animals for two different diagnosis methods.