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. 2020 Jan 10;40(1):BSR20191017. doi: 10.1042/BSR20191017

Figure 5. PAK1 is involved in the regulation of human OA chondrocytes phenotype by IL-1β.

Figure 5

(A and B) The protein expression of PAK1 was measured by Western blot in human OA chondrocytes transfected with siRNA-PAK1 for 24 h; n = 3, **P < 0.01. (C andD) The protein expressions of COL2α1, aggrecan, ADAMTS4 and MMP13 were measured by Western blot in human OA chondrocytes transfected with siRNA-NC or siRNA-PAK1, followed by treating with 10 ng/ml IL-1β for 24 h; n = 3, **P < 0.01. (E) The mRNA expression of COL2α1, aggrecan, ADAMTS4 and MMP13 were measured by qRT-PCR in human OA chondrocytes transfected with siRNA-NC or siRNA-PAK1, followed by treating with 10 ng/ml IL-1β for 24 h; n = 3, **P < 0.01. (F) The mRNA expression of COL2α1 and aggrecan was measured by qRT-PCR in human OA chondrocytes transfected with siRNA or overexpression plasmid; n = 3, *P < 0.05, **P < 0.01.