Figure 7. PAK1 activates inflammatory cytokines in rat chondrocytes stimulated with mechanical stress.
(A–F) The protein expressions of IL-1β (A and B), IL-6 (C and D) and TNF-α (E and F) were measured by Western blot in rat chondrocytes transfected with siRNA-NC or siRNA-PAK1, followed by treating with 10% mechanical stress for 24 h; n = 3, **P < 0.01, *P < 0.05. (G–I) The mRNA expressions of IL-1β, IL-6 and TNF-α were measured by qRT-PCR in rat chondrocytes transfected with siRNA-NC or siRNA-PAK1, followed by treating with 10% mechanical stress for 24 h; n = 3, **P < 0.01, *P < 0.05.