Table 3. Kinetic parameters for GH and transferase activity of C3larvinA.
| Parameter1 | GH | Transferase | |
|---|---|---|---|
| NAD+ | RhoA-GST | ||
| KM (µM) | 107 ± 20 | 75 ± 10 | 5.3 ± 1.0 |
| kcat (min−1) | 261 (±20) × 10−3 | 61 ± 3 | |
| kcat/KM (M−1.min−1) | 2.44 × 103 | 8.13 × 105 | 1.15 × 107 |
Kinetic parameters for transferase activity are shown for the RhoA target substrate.
1
The measurements of the kinetic parameters for C3larvinA GH and transferase activity are described in the ‘Experimental procedures’ section. The parameters represent the mean ± SD of at least three different measurements.