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. 2019 Dec 6;48(2):501–516. doi: 10.1093/nar/gkz1133

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Effect of Trp3 on the stability of the [4Fe–4S] cluster in the WhiB1:σ^A_CTD complex. (A) Solvent-accessible path from the surface towards the [4Fe–4S] cluster in the mutated model of the WhiB1-W3A:σ^A_CTD complex was calculated by CAVER 3.0 with a shell radius of 1.4 Å. The minimum bottleneck of the path is 1.7 Å. (B) Cell growth of M. smegmatis wildtype (WT) and the whiB1-W3A mutant on either a 7H10 agar plate for 3 days (top) or in 7H9 broth under optimal growth conditions. Error bars in the growth curves represent the standard deviation of triplicate samples.