Figure 2.

Characterization of SK-UT-1 cells knocked-out for HDAC4 and HDAC9. (A) Immunoblot analysis of HDAC9, MITR, HDAC4, HDAC5, MEF2A and MEF2D in SK-UT-1 cells WT and in two KO clones for HDAC4 (125 and 26) and HDAC9 (167 and D43). Asterisk points to a non-specific band. Actin was used as loading control. (B) Immunofluorescence analysis in SK-UT-1 WT, HDAC4^−/−, HDAC9^−/− cells stained with the indicated antibody. Where indicated, cells were treated for 2 h with Leptomycin B (Lept. 50 ng/ml) to inhibit the nuclear export. The anti-RAN antibody was used to stain nuclei. Bar: 50 μm.