Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 4;48(2):996–1009. doi: 10.1093/nar/gkz1123

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 3. — GlcN6P biosensors mediated transcription activation and repression. (A) The dose-dependent characteristics of the GlcN6P biosensors in strain BS03 (B. subtilis 168ΔgamRΔnagBΔgamA). GlcN was added into the media for improving the intracellular GlcN6P concentration. (B) The response of GlcN6P-responsive repression cascade constructed by the CRISPRi based NOR gate. (C) Verification of the function of the GlcN6P-responsive autonomous dual-control (ADC) system. Promoter P_sg2 was used both for the expression of mCherry and dCas9. Flow cytometry scatter plots shows GFP and mCherry fluorescence regulated by the ADC system, and the induction of the GlcN6P biosensor promotes the switch from the GFP⁺ to the mCherry⁺ state. The means of GFP and mCherry were calculated and marked in the figure. All data were the average of three independent studies with standard deviations.