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. 2019 Dec 4;48(2):788–801. doi: 10.1093/nar/gkz1126

Figure 3.

Figure 3.

G5-TPR lacks nucleic acids binding capacity. (A) Representative example of a gel-shift assay conducted with increasing amounts of purified G5-TPR (0 to 1 μM) and labeled domain 5 (d5) RNA. Purified RBS1 (0 to 1 μM) was used as a positive control of the RNA-binding assay. The percentage of retarded complexes obtained in three independent assays is plotted on the right panel. Values represent the mean ± SEM. (B, C) RNA-binding assays performed with G5-TPR and various probes: a 22 nt hairpin RNA, a 170 nt RNA predicted to fold in several stem-loops and a single stranded 32 nt RNA. (D, E) Lack of retarded complex formation of G5-TPR with ssDNA (29 nt) or ds DNA (29 bp).