Table 1.

Primer amplification characteristics of eight candidate genes for qRT-PCR in D. caesalis

Gene name (abbreviation)	Accession number	Primer sequence	Amplification size (bp)	Amplification efficiency (%)	Correlation coefficient (R2)
ACT	MN062588	5′ ACAATGAACTCCGTGTCGCC 3′ 5′ GTACATGGCGGGTGTGTTGA 3′	128	102.9	0.998
β-TUB	MN062589	5′ GGGAACGCTCCTCATCTCAA 3′ 5′ AGAGTGGCGTTGTAGGGTTC 3′	120	95.1	0.999
GAPDH	MN062590	5′ TCGTTGATCTCACTGTCCGC 3′ 5′ TTCGGTGTATCCAAGGACGC 3′	110	96.5	0.999
G6PDH	MN062591	5′ CCGATTTCCAACATCCGCAC 3′ 5′ CACTTTTCTGGCGCACATCC 3′	177	98	0.999
RPS3a	MN062592	5′ TGAACATGGCGGTCGGTAAA 3′ 5′ ATTGAACATGGACGGTGCCT 3′	125	99.2	0.999
RPL13a	MN062593	5′ CCGTGGACCTTTCCACTTCA 3′ 5′ ACTTCCAGCCGACTTCATGG 3′	245	107.5	0.998
EF1α	MN062594	5′ ATGGTTCAAGGGATGGCTCG 3′ 5′ GTTTCGACTCTGCCTACGGG 3′	180	98	0.998
EIF4A	MN062595	5′ ATGGGCCAAAGGATCAAGGG 3′ 5′ TGGGCTATAACATCGCGTCC 3′	211	106.4	0.998