Table 2.
Expression stability rankings of the eight candidate reference genes by three algorithms of geNorm, NormFinder, and BestKeeper
| Experimental conditions | Methods | Stability ranking | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
| Developmental stages | geNorm | ACT | RPS3a | EF1α | RPL13a | β-TUB | GAPDH | EIF4A | G6PDH |
| NormFinder | RPL13a | RPS3a | ACT | EF1α | GAPDH | EIF4A | β-TUB | G6PDH | |
| Bestkeeper | β-TUB | RPS3a | RPL13a | ACT | EF1α | EIF4A | G6PDH | GAPDH | |
| Tissues | geNorm | ACT | EF1α | RPS3a | EIF4A | G6PDH | GAPDH | RPL13a | β-TUB |
| NormFinder | ACT | RPS3a | EF1α | EIF4A | G6PDH | GAPDH | RPL13a | β-TUB | |
| Bestkeeper | EIF4A | ACT | EF1α | RPS3a | G6PDH | RPL13a | β-TUB | GAPDH | |
| Sexes | geNorm | ACT | EF1α | RPS3a | EIF4A | RPL13a | G6PDH | GAPDH | β-TUB |
| NormFinder | EF1α | RPS3a | EIF4A | ACT | RPL13a | G6PDH | GAPDH | β-TUB | |
| Bestkeeper | ACT | EF1α | RPS3a | EIF4A | β-TUB | RPL13a | GAPDH | G6PDH |