Table 3.
Primers and probes used in RT-PCR
| Genome Segment | Protein | Primer/probe sequence | Predicted product size (bp) | Reference |
|---|---|---|---|---|
| 7 | NSP3 |
NSP3-F: ACCATCTWCACRTRACCCTCTATGAG NSP3-R: GGTCACATAACGCCCCTATAGC NSP3-p: FAM5’-AGTTAAAAGCTAACACTGTCAAA-3’MGB |
87 bp | [32] |
| 6 | VP6 |
VP6F: GACGGVGCRACTACATGGT VP6R: GTCCAATTCATNCCTGGTGG |
380 bp | [33] |
| 2 | VP2 |
VP2F1: ACAAGCGGCAAATGACTGTT VP2R1: GGTTGATGACTTGTCCACTCC |
398 bp | This paper |
| 11 | NSP5 |
NSP5F1: ACAACGTCAACTCTTTCTGGA NSP5R1: TGCTTGAAGGTCGTGATTGC |
232 bp | This paper |
| SISPA |
FR26RV-N: GCCGGAGCTCTGCAGATATCNNNNNN FR20RV: GCCGGAGCTCTGCAGATATC |
[34] |