Fig. 3.

Transcription is not required for upregulation of toxicity-associated miRNAs. a, b QRT-PCR analysis of miRNA expression 4 h after stimulation with NMDA (30 μM) in the presence or absence of the transcription inhibitors alpha-Amanitin (a) or actinomycin D (b). Mean values + SEM from ≥3 independent experiments are shown. P-values were determined by repeated-measures ANOVA with Tukey’s post test (a) and two-tailed paired t test (b). c QRT-PCR analysis of miR-132 expression 4 h after stimulation with bicuculline (Bic, 50 μM) in the presence or absence of the transcription inhibitor alpha-Amanitin. Mean values + SEM from 4 independent experiments are shown. P-value was determined by two-tailed t test