Table 2.
Most representative Bracon nigricans venom proteins
| Transcript ID | RPKM | Band | Best hit in SwissProt db | InterProScan Result | Species having putative homologs |
|---|---|---|---|---|---|
| comp24796_c0_seq1* | 11,689.63 | 27 | No hits found | PBP/GOBP family - Odorant Binding Protein | Ac, Ci, Nv, Lh, Pp |
| comp22364_c1_seq2* | 9209.46 | 26 |
Phospholipase A2 (PA3A/PA3B/PA5) P16354 Heloderma suspectum |
Phospholipase A2 | Bh, Eo, Pc, Tn |
| comp17530_c0_seq1 | 4073.65 | 27 | No hits found | Prokaryotic membrane lipoprotein lipid attachment site profile | Bh |
| comp24797_c0_seq1* | 3996.5 | 11, 12 |
Venom carboxylesterase-6 B2D0J5 Apis mellifera |
Carboxylesterase, type B | Ac, Dc, Hd, Nv |
| comp24806_c0_seq1 | 3088.82 | 23 |
Venom allergen 5 P86870 Vespa magnifica |
Venom allergen 5-like - CRISP | Ci, Hd, Lh, Mh, Nv, Tb |
| comp24842_c0_seq1 | 436.95 | 10, 11 | No hits found | DUF4803 | Cd, Mh, Pc, Pl |
| comp14282_c0_seq1 | 398.38 | 7 | No hits found | DUF4803 | Cd, Mh, Pc, Pl |
| comp16818_c0_seq2* | 288.36 | 20 |
Chymotrypsin-1 Q27289 Anopheles gambiae |
Serine protease, peptidase, chymotrypsin | Ae, Bh, Ci, Cr, Es, Hd, Nv, Ph, Pp, Tn |
| comp20898_c0_seq1 | 280.35 | 10 | No hits found | DUF4803 | Cd, Mh, Pc, Pl |
| comp18097_c0_seq1 | 224.37 | 27 | No hits found | PBP/GOBP family - Odorant Binding Protein | Ac, Ci, Nv, Lh, Pp |
| comp20936_c0_seq1 | 180.95 | 9 | No hits found | DUF4803 | Cd, Mh, Pc, Pl |
| comp13820_c0_seq1* | 156.34 | 6, 7 |
Aminopeptidase M1-A Q6Z6L4 Oryza sativa |
Aminopeptidase N-type | Cc, Lb, Ph, Pl |
| comp24916_c0_seq1 | 117.70 | 9, 10 | No hits found | DUF4803 | Cd, Mh, Pc, Pl |
| comp21345_c1_seq3* | 102.09 | 13 |
Protein disulfide-isomerase P54399 Drosophila melanogaster |
Disulfide isomerase - PDI | Ae, Cc, De, Pc, Pl, Pp |
| comp24941_c0_seq1 | 95.31 | 13 |
Platelet glycoprotein V O08742 Mus musculus |
Leucine Rich Repeat | Ae, Pc, Pl |
| comp22420_c0_seq1* | 90.06 | 16 |
Lipase 3 O46108 Drosophila melanogaster |
Lipase | Ci, Lb, Ma, Nv, Ot, Ph, Pp |
| comp22102_c0_seq1 | 79.94 | 11 | No hits found | DUF4803 | Cd, Mh, Pc, Pl |
| comp22165_c0_seq2* | 71.83 | 5 |
Lysosomal alpha-mannosidase Q8VHC8 Cavia porcellus |
GH38 - Alpha mannosidase | De |
List of proteins identified by SDS-PAGE and LC-MS/MS of crude venom extract, sorted by descending order of RPKM values, filtered for the presence of the signal peptide and highly represented in the venom gland transcriptome (i.e., in the upper third of RPKM values distribution). *Candidates selected for expression studies. Ac, Anisopteromalus calandrae. Ae, Aphidius ervi. Bh, Bracon hebetor. Ci, Chelonus inanitus. Cc, Cotesia chilonis. Cr, Cotesia rubecula. Dc, Dinocampus coccinellae. De, Diversinervus elegans. Eo, Eupelmus orientalis. Es, Euplectrus separatae. Hd, Hyposoter didymator. Lb, Leptopilina boulardi. Lh, Leptopilina heterotoma. Ma, Microctonus aethiopoides. Mh, Microctonus hyperodae. Nv, Nasonia vitripennis. Ot, Ooencyrtus telenomicida. Ph, Pimpla hypochondriaca. Pp, Pteromalus puparum. Pc, Psyttalia concolor. Pl, Psyttalia lounsburyi. Tb, Tetrastichus brontispae. Tn, Toxoneuron nigriceps. References for the homologs of B. nigricans venom proteins present in other parasitoid species are shown in Additional file 4: Table S3