. 2020 Jan 10;21:18. doi: 10.1186/s12891-019-3031-0

Table 1.

Characteristics of polymorphisms by measurement methods

SNP	Location	Nucleotide position	Analysis method	Enzyme	Nucleotide variants	Primer’s sequence 5′➔3′	Annealing temperature
rs78874766	Intronic	60,777,684	Sequencing	n/a	A/G	CATCGGGTCTCCTTTGGTTA	58 °C
rs4738824		60,777,762			A/G	ATCAATTCCCACTGCCTGAC
rs74797613		60,777,857			A/G	ATCAATTCCCACTGCCTGAC
rs13248429	Exon 2	60,742,360	RFLP	NcoI	A/T	CAGCAGATGGGCAGCTATATG
rs13248429	Exon 2	60,742,360		NcoI	A/T	ATTAGTCCTTGACCACTGTTTGATG
rs1017861	Intronic	60,706,154		EcoRI	A/G	GTCCTGAGCTATGCAGTTCCA	61 °C
rs1017861	Intronic	60,706,154		EcoRI	A/G	ACCCCTTGTTCTTTCATCACCT	61 °C
rs4738813	Intronic	60,683,112		DpnII	C/T	GCAAGCCTTCCATGGTTAAA	65 °C
rs4738813	Intronic	60,683,112		DpnII	C/T	AGCTATGGACCTCTGCCATC	65 °C

Abbreviations: SNP single nucleotide polymorphism, RFLP restriction fragment length polymorphism, n/a not applicable