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. 2020 Jan 10;51:3. doi: 10.1186/s13567-019-0730-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Vaccinated piglets exhibited significant levels of serum IgG against Salmonella at the time of challenge. Sera from the control and vaccinated group were pooled into four samples per day. A Sera were analyzed by Western blot against a hot saline antigenic extract of Salmonella ΔXIII strain. In this case, an equal volume of each pool and group was mixed and the resulting samples were used for analysis. C: control, V: vaccinated. B Pooled sera were analyzed by an LPS based IDEXX test. An S/P ratio of ≥ 0.25 is considered positive. Statistical analysis was carried out using a two-way analysis of variance combined with the Bonferroni test. **P < 0.01.