Fig. 2.

PCR amplification of biofilm-encoding genes in one selected clinical isolate of P. aeruginosa as representative. a Lane 1–3: PCR products of the pelF, algD, and pslD genes, respectively. M: 50 bp DNA ladder. Lane 4: PCR mixture without DNA template as control negative. Lane 5–7: PCR products of the corresponding genes in P. aeruginosa PAO1 reference strain as control positive. b A 50 bp DNA ladder containing seventeen discrete fragments ranging from 50 to 1500 bp with double intensity reference bands at 200 bp, 500 bp, and 1200 bp