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. 2020 Jan 11;13:5. doi: 10.1186/s13068-019-1643-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Effect of metals on thermostability of PirXI. The graphs show the apparent melting temperatures of purified and EDTA-treated wild-type PirXI (green lines) and the V270A–A273G mutant PirXI (orange lines) in the presence of various concentrations of metals (a Mn²⁺; b Mg²⁺; and c Ca²⁺). The T_m values are averages from two independent measurements. [E] = 20 μM. Error bars represent standard deviations