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. 2019 Oct 29;199(2):150–162. doi: 10.1111/cei.13382

Figure 6.

Figure 6

Internalization of 2C10 and DNA induces expression of interferon (IFN)‐α and proinflammatory cytokines by peripheral blood mononuclear cells (PBMCs). (a) PBMCs were incubated with 5 μg/ml 2C10 or isotype control (IC) for 4 or 48 h, with or without prior 10‐min incubation with 200 ng/ml unlabeled DNA, and cytokine levels in the supernatants were assessed. Data represent the mean ± standard error of the mean (s.e.m.) of four to seven experiments using PBMCs from two to three donors. (b) PBMCs were incubated with 5 μg/ml 2C10 or IC for 4 h, with or without prior 10‐min incubation with 10 ng/ml unlabeled DNA. Data represent the mean ± s.e.m. of three experiments using PBMCs from a single donor. (–): no stimulation; 2 + D: 2C10 + DNA; IC + D: isotype control + DNA. Every figure was analyzed by a one‐way analysis of variance (anova) followed by Tukey’s multiple comparison test, and only significantly different pairs are indicated by asterisks. *P< 0·05; **P < 0·01; ***P < 0·001.