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. 2019 Oct 24;199(2):201–215. doi: 10.1111/cei.13377

Figure 5.

Figure 5

Mucosal‐associated invariant T (MAIT) cell abundance in sputum is associated with the number of neutrophils in sputum. The abundance of (a) MAIT cells (Vα7.2‐Jα12/20/33 gDNA) and (b) Vδ2+ T cells (Vδ2‐Jδ1/2/3/4 gDNA) by the number of neutrophils (PMN) per low‐powered field (LPF) in sputum. MAIT cell and Vδ2+ T cell abundance was determined by quantitative polymerase chain reaction (qPCR); the absolute copy number of β2 microglobulin (β2M) was calculated with LinRegPCR 23 and the number of MAIT cells (Vα7.2‐Jα12/20/33 gDNA) and Vδ2+ T cells (Vδ2‐Jδ1/2/3/4 gDNA) determined relative to β2M and expressed in arbitrary units. Microscopy was performed on sputum in the diagnostic laboratory prior to freezing. Individual data points (n = 52) representing the mean of technical triplicates of the qPCR assay, the median and interquartile ranges are shown. Data were analysed with the Kruskal–Wallis one‐way analysis of variance (ANOVA); n.s. = not significant.