Figure 6.

Interaction between FAM181A/B and TEAD4 in cells. N‐terminally V5‐tagged wtTEAD4 or Asp272Ala^TEAD4 were co‐transfected with (FLAG)₃‐FAM181A/B into HEK293FT cells. TEAD4 was immunoprecipitated with a V5 specific antibody and co‐immunoprecipitated FAM181A/B was determined by anti‐FLAG Western blot. Left panel co‐transfection FAM181A:TEAD4. Right panel co‐transfection FAM181B:TEAD4. To provide a better view without signal saturation, a shorter exposure of total FAM181B is shown (flag in the lysates sub‐panel). IP, Immunoprecipitated fraction