Figure 2.

The conventional two‐band genotyping PCR strategy does not distinguish mice with germline‐recombined alleles. (a) Schematic diagram of the conventional two‐band genotyping PCR primers illustrating that mice with a germline‐recombined (rcb) Insr allele would yield no PCR product and that Insr^wt/wt and Insr^wt/rcb mice would appear identical as would Insr^fl/fl and Insr^fl/rcb mice. (b) Punnett square with a hypothetical F₃ cross, in which Foxp3^YFP‐CreInsr^fl/rcb mice (mistaken as Foxp3^YFP‐CreInsr^fl/fl) are crossed with Foxp3^YFP‐CreInsr^fl/wt mice (left). Table compares hypothetical and actual F₄ genotype ratios (right). n = 62 in the F₄ generation.