Figure 4.

A quantitative PCR strategy quantifies the extent of unwanted ectopic recombination in gDNA. (a) Schematic diagram of the location of three sets of qPCR primers specifically designed to amplify one Insr allele: wild‐type, floxed or recombined. (b) Quantitative PCR was performed with ear‐notch tissue lysates with these three primer pairs. The relative amounts of each allele were quantified and normalized to those of a Foxp3^YFP‐Cre mouse, an Insr^fl/fl mouse or a Foxp3^YFP‐CreInsr^wt/rcb mouse. Each column represents one mouse; data are representative of two independent experiments.