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. 2020 Jan 7;13:131–142. doi: 10.2147/OTT.S217335

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© 2020 Wang et al.

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BAMBI negatively interacted with miR-17-5p in A549 and H1299 cells. (A) The complementary binding sites between miR-17-5p and BAMBI were shown. (B–C) The luciferase activity of BAMBI 3ʹUTR WT or BAMBI 3ʹUTR MUT reporter in A549 and H1299 cells transfected with miR-17-5p mimics or miR-NC were assessed by dual-luciferase reporter assay. (D–G) The H1299 and A549 cells were transfected with miR-control, miR-17-5p, inhibitor-control or miR-17-5p inhibitor. (D–E) The mRNA level of BAMBI was measured by qRT-PCR. (F–G) The protein level of BAMBI was tested via Western blot assay. *P < 0.05.