Figure 3.

Characterization of crude reaction mixes and purified products for HIR-FH labeled with different radiocations. (A) The radiochemical yields (RCY) by SEC (PD-10 column) for reaction mixes yielding [⁹⁰Y]Y-FH or [¹⁷⁷Lu]Lu-FH are shown. Deferoxamine (DFO) is added to remove loosely bound radiocations from FH, and to ensure that radiocations are not present as oxides, but as low molecular weight, radio-DFO complexes. These complexes migrate at the included column volume in SEC or stay at the origin in TLC. See legend for 3e below. All values are decay corrected. Integrated areas under the peaks were used to obtain RCY or RCP. (B) Radiochemical purity (RCP) by SEC after reaction mixes from (A) was purified by SEC. (C) RCY by SEC for reactions yielding [⁶⁴Cu]Cu-FH is shown. Because the RCY for [⁶⁴Cu]Cu-FH (68.1%) was lower than that seen with [⁹⁰Y]Y-FH or [¹⁷⁷Lu]Lu-FH (90.1% or 95.1% in Figure 3a), variants of the standard HIR procedure, the HRR-HIR and HV-HRR-HIR procedures, were developed. (D) RCP by SEC after reaction mixes from (C) was purified by SEC. (E) RCY by TLC for reaction mixes yielding [⁹⁰Y]Y-FH or [¹⁷⁷Lu]Lu-FH is shown. With TLC, radio-DFO complexes remain at the origin (arrows) while negatively charged radio-FH NPs move to the solvent front on our negative charged, cation exchange TLC plates. (F) RCP by TLC after reaction mixes from (E) was purified by SEC. (G) RCY by TLC for reactions yielding ^64CuCu-FH. (H) RCP by TLC after reaction mixes from (G) was purified by SEC.