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. Author manuscript; available in PMC: 2020 Jun 23.
Published in final edited form as: Nat Struct Mol Biol. 2019 Dec 23;27(1):42–48. doi: 10.1038/s41594-019-0352-5

Fig. 4: Disulfide-crosslinked CTPS2 filaments can switch conformations.

Fig. 4:

(a) CTPS2 filament showing the position of the CTPS2CC mutant at the filament interface. (b) Zoomed-in view of the orange box in (a), showing the design of the CTPS2CC mutant. The V352C mutation is across from native C362. (c) Negative stain EM images of hCTPSCC under reducing conditions (+DTT), and under non-reducing conditions in the presence or absence of nucleotides. Scale bars are 50 nm. (d) 2D averages of S- and P-state CTPS2CC filaments, with characteristic 180° repeat distances indicated. (e) 2D class averages of cross-linked CTPS2CC filaments before and after addition of CTP. 2D averages (A) are aligned to low-pass filtered projections of CTPS2 cryo-EM models (M) in the S-state (top) and P-state (bottom) conformations.