. Author manuscript; available in PMC: 2020 Jun 23.

Published in final edited form as: Nat Struct Mol Biol. 2019 Dec 23;27(1):42–48. doi: 10.1038/s41594-019-0352-5

Table 1:

Cryo-EM data collection, refinement and validation statistics

	S state CTPS2 filament (EMD-20354, PDB 6PK4)	P state CTPS2 filament (EMD-20355, PDB 6PK7)
Data collection
Magnification	130000	130000
Voltage (kV)	300	300
Electron fluence (e^–/Å²)	90	90
Defocus range (μm)	1.0–2.5	1.0–2.5
Pixel size (Å)	0.525	0.525
Reconstruction
Pixel size (Å)	1.05	1.05
Symmetry imposed	D2	D2
Helical symmetry
Rise (Å)	101.5	101.5
Rotation (°)	55.3	41.7
Particle images (no.)	53964	22705
Map resolution (0.143 fsc) (Å)	3.5	3.1
Refinement
Initial model used (PDB code)	2V4U, 2VO1	2V4U, 2VO1
Map sharpening B factor (Å²)	−50	−50
Model composition
Protein residues	559	557
Ligands	UTP, ATP	CTP, ADP
R.m.s. deviations
Bond lengths (Å)	0.41	0.52
Bond angles (°)	0.69	0.66
Validation
MolProbity score	2.15	2.06
Clashscore	12	11
Poor rotamers (%)	1	0.4
Ramachandran plot
Favored (%)	92	91
Allowed (%)	8	9
Outliers (%)	1	0