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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Nov 15;1866(3):165608. doi: 10.1016/j.bbadis.2019.165608

Figure 6. Control EVs exhibit biological effect when provided with annexin A2 required for uptake.

Figure 6.

EVs were collected from annexin A2 lentiviral vector transfected cells. The expression vector was designed to target annexin A2 to the surface of EVs. (A) Elevated levels of annexin A2 in control EVs could be confirmed by Western blotting upon transfection with varying concentrations of lentiviral vectors (infectious units/mL). (B) EVs from untransfected stressed cells reduce TER, whereas those from untransfected control cells do not. In contrast, EVs from transfected control cells do reduce TER to the same level as stress EVs; and the addition of stress in transfected cells does not enable further reduction of TER upon EV transfer. Graphs represent mean ±SD (n=3-5 independent Western blots; and n=4-8 wells, 6 locations each for TER). Significance was obtained using a T-test (Western) or posthoc ANOVA with Bonferroni correction (TER assay). Western analysis was significant (P<0.001). * indicates difference of indicated TER values with those elicited by control EVs.