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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Immunity. 2019 Nov 12;51(6):1088–1101.e5. doi: 10.1016/j.immuni.2019.10.004

Figure 3. Localized proliferation and SHM of B cells in the spleen and liver.

Figure 3.

EdU positive B cell blasts (A) and PB (B) of spleen (blue) and liver (red) over the course of infection measured by FC. (C-D) 40X image of proliferating B cells in a naïve spleen (C) and E. muris infected spleen at D10 post infection (D). (E, F) 10X (E) and 40X (F) images of proliferating B cells in the liver parenchyma at D10 post infection. In C and F, scale bars represent 20μm, in D scale bars represent 200 pixels and in E, scale bars represent 100μm. (G-H) Percentage of mutated nucleotides in PB from spleen (G) and liver (H). (I-J) Laser microdissections of IgM-positive PB patches (green) and corresponding clonal trees from Ig region sequencing from the spleen (I) and the liver (J). Inferred nodes are blue. Node size is proportional to the number of sequences. The CDR3 amino acid sequence of each clone shown at the bottom. Data are representative of at least two independent experiments. See figure S3 and table S1.