. 2020 Jan 12;6(1):vez058. doi: 10.1093/ve/vez058

Table 1.

Primer sequences used to recover the full genome, pol, and env proviral sequences.

Primer name	Nested round	F/R primer	Product size of final amplicon	Primer sequence
Full FFV genome—5′ half	1	F	∼6.3 kb	GAATTCTCACAGAGGAGAATACTCTCTGC
	1	R		GCAACTCAGGATGAGTCAACTGAAGTTTCTG
	2	F2		GTCAACAAAAGCTCTTTTATCCCGGAG
	2	R2		TGGCCTAGATGGTCCACTATAATCACA
Full FFV genome—3′ half	1	F	∼6.3 kb	GTTGGAGGGAAATTCCTCCTTCCCGAG
	1	R		ACTGTCGTGGTCTATACCTGGGATAGGTTAG
	2	F2		AGTCATGCAAGACGAGAAGCCGT
	2	R2		CACTTTTCCCCAGGAATAGAGAAACAC
FFV env gene	1	F	∼3.2 kb	CTAAGTGGAGGAAGCCTACAAAGG
	1	R		CCACTTTCAGGAATTCCCTTATGACATTG
	2	F2		GATTATAGTGGACCATCTAGGCCAAC
	2	R2		CTGGTATGCATAGACAAAAGAGCTAAAG
FFV pol gene	1	F	∼3.9 kb	GTAATCCTCAACAGCAGGGACC
	1	R		GGAAGTATTTCCTCCCACGGTTAC
	2	F2		ATCAAGGACCTCGGCCAG
	2	R2		CAGGATGTGTTATTGCTTCTTTCCATTG