Table 1. Cryo-EM data collection, refinement and validation statistics.
| 165bp H3KC36me3 nucleosome-LEDGF (EMD-10069, PDB 6S01) | |
|---|---|
| Data collection and processing | |
| Magnification | 130,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 43.2 |
| Defocus range (μm) | –1.25 to –2.75 |
| Pixel size (Å) | 1.05 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 527,640 |
| Final particle images (no.) | 55,142 |
| Map resolution (Å) | 3.2 |
| FSC threshold | 0.143 |
| Map resolution range2 (Å) | 3.0-5.8 |
| Refinement | |
| Initial models used (PDB code) | 3MVD, 4FU6 |
| Model resolution (Å) | 3.1 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | -- |
| Map sharpening B factor (Å2) | -124 |
| Model composition | |
| Non-hydrogen atoms | 12959 |
| Protein residues | 853 |
| Nucleic acid residues | 300 |
| B factor (Å2) | |
| Protein | 88.0 |
| Nucleic acid | 142.7 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.01 |
| Bond angles (°) | 0.73 |
| Validation | |
| MolProbity score | 1.44 |
| Clashscore | 3.93 |
| Poor rotamers (%) | 0.42 |
| Ramachandran plot | |
| Favored (%) | 96.13 |
| Allowed (%) | 3.87 |
| Disallowed (%) | 0 |