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. 2019 Dec;31(6):955–964. doi: 10.21147/j.issn.1000-9604.2019.06.11

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Copyright © 2019 Chinese Journal of Cancer Research. All rights reserved.

This work is licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Construction, transfection and identification of plasmids with downregulated inter-α-trypsin inhibitor heavy chain 4 (ITIH4). (A) ITIH4 gene was expressed in A2780, SKOV3, HO8910pm, HO8910 ovarian cancer cells with the highest expression in HO8910pm cells; (B) ITIH4-917 siRNA fragment had a maximal ITIH4-silencing rate of 87.2%. This fragment was selected to construct the ITIH4-silencing plasmid pGPU6/GFP/Neo-shRNA-ITIH4-917, and then transfected into HO8910pm cells with 60% transfection efficiency; (C) (a) represents the monoclonal pGPU6/GFP/Neo-shRNA-ITIH4-917-HO8910pm cells screened by G418 (800 μg/mL) selection after 14 d. (b−d) represent the state of monoclonal cells after 3 d, 7 d, and 14 d of culture, respectively. ×200.