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. 2019 Dec;31(6):955–964. doi: 10.21147/j.issn.1000-9604.2019.06.11

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Copyright © 2019 Chinese Journal of Cancer Research. All rights reserved.

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Confirmation of inter-α-trypsin inhibitor heavy chain 4 (ITIH4) knockdown. (A,B) Results of quantitative reverse transcription polymerase chain reaction (RT-qPCR) indicated that expression of ITIH4 mRNA in pGPU6/GFP/Neo-shRNA-ITIH4-917-HO8910pm cells was lower than that in pGPU6/GFP/Neo-shRNA-ITIH4-NC-HO8910pm and pGPU6/GFP/Neo-shRNA-HO8910pm cells. *, P<0.05; (C) Western blot indicated that the expression of ITIH4 protein in pGPU6/GFP/Neo-shRNA-ITIH4-917-HO8910pm cells was lower than that in pGPU6/GFP/Neo-shRNA-ITIH4-NC-HO8910pm and pGPU6/GFP/Neo-shRNA-HO8910pm cells; the difference was statistically significant (P₁=0.008, P₂=0.014). 1, Expression of ITIH4 protein in pGPU6/GFP/Neo-shRNA-ITIH4-917 group; 2, Expression of ITIH4 protein in pGPU6/GFP/Neo-shRNA-ITIH4-NC group.