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. 2019 Dec;31(6):955–964. doi: 10.21147/j.issn.1000-9604.2019.06.11

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Role of inter-α-trypsin inhibitor heavy chain 4 (ITIH4) knockdown in cell growth, cell cycle, migration and invasion. (A) Cell growth was assessed by MTT. Blue line represents the growth curve of pGPU6/GFP/Neo-shRNA-ITIH4-NC; red line represents the growth curve of pGPU6/GFP/Neo-shRNA-ITIH4-917 and the purple line represents the growth curve of pGPU6/GFP/Neo-shRNA. Cell growth rates were increased in pGPU6/GFP/Neo-shRNA-ITIH4-917-HO8910pm cells compared with the other two groups; (B) Cell phase of S+G2+M was significantly increased in pGPU6/GFP/Neo-shRNA-ITIH4-917-HO8910pm cells. The rate of S+G2+M phase cells between the other two groups was no difference. *, P<0.05; (C) Cell migration abilities. a, pGPU6/GFP/Neo-shRNA-ITIH4-917-HO8910pm cells; b, pGPU6/GFP/Neo-shRNA-ITIH4-NC-HO8910pm cells; c, pGPU6/GFP/Neo-shRNA-HO8910pm cells. Compared a with b, P<0.05; Compared a with c, P<0.05; Compared b with c, P>0.05. *, P<0.05.