Fig. 7.
Wnt16 function in mesoderm morphogenesis and gastrulation. (A) 1d5 and Meso1 antibody staining at mesenchyme blastula stage (24 hpf). 1d5 (blue) stains skeletogenic mesoderm cells, and Meso1 (orange) is a general mesoderm marker. Neither 1d5 nor Meso1 staining was affected in Wnt16 knockdown embryos (Ae-h) compared with control embryos (Aa-d). (B) F-actin staining as measured by phalloidin binding. F-actin accumulation was present in the invagination of both control and Wnt16 knockdown embryos (Ba-d). MO, morpholino.