Fig. 5.

MiR-125b was negatively correlated with SIRT7. a A binding site at 3′-UTR of SIRT7 was predicted on miR-125b by StarBase3.0. b The luciferase activity was measured by dual-luciferase reporter gene assay. c Relative mRNA expression of SIRT7 in ischemia reperfusion injury (I/R) myocardium tissues was examined by qRT-PCR. d Spearman’s correlation analysis was used to detect the correlation between miR-125b and SIRT7 expression. e, f qRT-PCR and Western blot were used to detect the mRNA and protein expression of SIRT7 in myocardium cells