FIG 11.

Ifit2 is upregulated in mouse corneas infected with HSV-CD80. RNA was isolated from corneas of uninfected, HSV-CD80-infected, or parental virus-infected corneas. qRT-PCR was performed using total RNA. (A) Expression of CD80, Gvin1, Mip, Ifit1, and Ifit2 in naive mice was used to estimate the relative expression of each transcript in cornea, and GAPDH was used to normalize the expression of each transcript. Each bar represents the mean from 30 TG ± the SEM. Results were considered significant if P < 0.05 (Student t test). (B) The estimated relative copy number of the HSV-1 gB transcripts was calculated using standard curves generated from pAc-gB1. Each bar represents the mean from 30 TG, and error bars represent the SEM (P = 0.25 [Student t test]).