FIG 2.

Levels of replication of HSV-CD80 virus and parental virus in mouse corneas are indistinguishable. (A) Corneas of female BALB/c mice were ocularly infected with 10⁵ PFU/eye HSV-CD80 or parental virus and harvested on days 3 and 5 p.i. The gB copy number was determined by qPCR. No differences in gB copy number were seen between the two groups (P = 0.4 and P = 0.7 [Fisher exact test] on days 3 and 5 p.i.). (B) Virus titers were determined from tears of mice infected with either HSV-CD80 or parental virus on days 1 to 7 p.i. Viral titers peaked around days 2 to 3 p.i., and virus was cleared from tears by day 7 p.i. No significant differences were seen in titers from mice infected with HSV-CD80 or parental virus (P > 0.05 [Fisher exact test]). Error bars represent the SEM.